ABSTRACT
Aim: The filariasis infection is initiated by mosquito derived third stage larva (L3), which establishes itself in different immunocompetent niches by adopting different evasion and immunomodulatory mechanisms. Immunological and clinical outcomes can vary considerably at the individual and population levels during lymphatic filariasis infection. The protein product coded by the interleukin-10 (IL-10) gene has broad immunomodulatory function in filarial load and patency of the disease. The potential influence of altered IL-10 expression encoded by IL-10 promoter single nucleotide polymorphisms (SNPs) and IL-10RA signaling pathway, in pathogenesis and clinical outcome of filarial infection was established in the present study Study Design: Genetic association based on case-control study. Place and Duration of Study: Lymphatic filariasis cases referred to National Filariasis Control Program (NFCP), Siddipet, Medak, Andhra Pradesh, India between Feb 2006 to Dec 2009. Methodology: A total of 100 non-endemic, 50 endemic and 118 lymphatic filariasis patients were included in the present study based on clinical and diagnostic criteria. Genetic polymorphisms in the IL-10 promoter region (-1082G/A, -819C/T and -592 A/C) and IL-10 RA coding region S138G were screened following PCR-RFLP and ARMS-PCR technique respectively. Results: Patients with familial aggregation of lymphedema exhibited significant association with IL-10 -1082 ‘A’ allele (A vs G OR 2.68, CI - 1.12-6.37, P=0.02) coding for lower IL-10 levels. Similarly the G variant of IL-10RA S138G SNP revealed a significant association with lymphatic filariasis in the endemic population studied (GG vs AA OR 2.50 CI-1.22-5.13, P= 0.021). The Haplotype analysis also revealed the low signaling ATA is significantly associated with the disease in this cohort (P=0.03). The Multifactor Dimensionality Reduction Analysis (MDR) for IL-10 and IL-10RA SNPs interaction revealed the three locus model as the best model wherein the epistatic interactions of variant G allele of IL-10RA S138G, the A allele of the -1082G/A and the T allele of the -819C/T SNPs in IL-10 were found to be a possible risk genotype for filarial infection. (TA = 0.5230, CV-10/10, P=0.001). Conclusion: IL-10 promoter haplotypes and IL-10 RA S138G polymorphisms are the possible genetic determinants of susceptibility to lymphatic filariasis. Further functional studies are warranted to validate these results.
ABSTRACT
A total of twenty five endophytic bacteria were isolated from green gram Vigna radiata (L.) plant. Four isolates which have shown better response for plant growth promoting effects on green gram plants were used as Plant Growth Promoting Bacteria (PGPB) in this study. It has been detected that up to 40% of the genomic 16S rRNA of isolated bacteria belongs to the Azotobacter genus. The result for phosphate solubilizing activities in NPRIB broth and Pikovskaya broth by UV-VIS Spectrophotometer (830 nm) showed that isolate FR was the best candidate in solubilizing of phosphorous showing 49.817Ppm with NPRIB broth and 53.534Ppm with Pikovskaya broth,respectively. Of all studied endophytes, isolates NL and NR have shown high nitrogenase activities and the activities being 2570.40 and 2108.00 n mole of C2H4 ml-1h-1 respectively. These strains will be selected for further use. The IAA production of all isolates was identified by FTIR spectroscopy and Spectrum for Crude IAA Extract of isolate FL is the best candidate for IAA production. The insoluble Potassium (K+) content in mica sample and the soluble K+ content by the inoculated samples were analyzed the atomic absorption spectrophotometric (AAS) method and isolates FL, FR, and NR were found to be good at decomposing insoluble potassium. These results indicated that the selected all four endophytic microbes could be excellent candidates for use as biofertilizer.